Inhibitory effect of silver oxide nanoparticles and Saccharomyces cerevisiae on Escherichia coli and Staphylococcus aureus bacteria
DOI:
https://doi.org/10.56053/10.1.13Keywords:
Saccharomyces cerevisiae, Silver oxide, Nanoparticles, Staphylococcus aureusAbstract
Baker's yeast, or Saccharomyces cerevisiae, is a unicellular fungus that has been extensively studied and used as a suitable biofactory for the production of bio nanoparticles. The study aims to investigate the inhibitory effect of silver oxide nanoparticles on pathogenic bacteria. This study examines the biosynthesis, characterization, and antibacterial efficacy of silver oxide nanoparticles (AgO-NPs) that
are synthesized from Saccharomyces cerevisiae extract. Pathogenic bacteria, specifically Staphylococcus aureus and Escherichia coli, are isolated and identified from diarrhoea samples and utilized as test organisms. Phytase enzyme is isolated from both commercial and locally sourced S. cerevisiae yeast using solvent extraction and precipitation techniques. The isolated enzyme functioned
as a biocatalyst in the eco-friendly creation of AgO nanoparticles. Silver oxide is reduced and stabilized using the enzymatic extract, resulting in nanoparticles that are later characterized by FTIR, UV–vis spectroscopy, and scanning electron microscopy (SEM). FTIR examination verified the existence of functional groups, including hydroxyl, carbonyl, and amine groups, signifying the participation of
proteins, phenols, and polysaccharides in the stabilization of nanoparticles. The UV–vis spectra exhibited a large absorption peak at 400–430 nm, indicative of surface plasmon resonance (SPR) in silver nanoparticles, whereas SEM micrographs displayed spherical, agglomerated nanoparticles with an average diameter of 53 nm. The antibacterial activity is evaluated using the well-diffusion method
against Staphylococcus aureus and Escherichia coli. The AgO-NPs demonstrate concentration dependent inhibition, achieving maximum inhibition zones of 15 mm and 16 mm, respectively, at 100 mg/dl. The results indicate the promise of yeast-derived silver oxide nanoparticles as environmentally sustainable and efficient antibacterial agents against pathogenic microorganisms.
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