Nanostructured flax seeds-based fatty acids effect on genotype of Leishmania tropica

Abstract

The effect of fatty acids extracted from flax seeds (Linum usitatissimum) on the genotype of Leishmania tropica concentrations 100 micrograms/deciliter dl are prepared by means of dissolving fatty acids with Ethylen glycol to attain the above concentrations. These fatty acids had been used as capacity antilieshmanial retailers in vitro, and the results showed that the awareness of 100 micrograms/deciliter gave an entire inhibition of the parasite boom in the promastigote form in 48 hours and as much as one hundred% as compared to the manage group that witnessed the parasite growth at all times. In this study, 8 non-specific primers are used (table below), and these primers search for mutations in the entire genome, both coding and non-coding, and the use of these primers is the first time on the Leishmania parasite. Molecular study on the parasite DNA using Random Amplified Polymorphic DNA Polymerase Chain Reaction (RAPD-PCR) technique has proven the ability of these fatty acids to cause lethal mutations in the parasite. The 0.5 % concentration of fatty acids is used in the treatment of patients infected with cutaneous leishmaniasis, and it led to their
complete recovery within 10 days without leaving a scar, compared to the group of patients treated with Pentostam who needed 6 weeks to recover. Scanning electron microscopy (SEM) has been employed to characterize this work before and after fatty acids effect, followed by analysis of absorption and Fourier transform infrared spectroscopy (FTIR).